TTAGGG ), ODN TTAGGG control and Imiquimod were from Invivogen. Differentiation of pDCs from Mouse Bone Marrow and Fetal Liver Cells. Bone marrow cells from the femur and tibia of adult C57BL/6 mice and fetal livers from pups
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چکیده
Antibodies and Reagents. Recombinant mouse IFN-γ was from PeproTech, recombinant human IFN-β was from PBL Biomedical, recombinant mouse IFN-β was from R&D Systems and recombinant human Flt-3L was from Origene Inc. Anti-CD32a (clone IV.3) was from Stemcell Technologies, Alexa-Fluor-647 conjugated anti-LAMP1 (clone eBio1D4B) was from eBioscience, APC-conjugated anti-B220 (clone R3-6B2) was from Biolegend, PEconjugated anti-CD11c (clone HL3) was from BD Pharmigen and anti-TNF-α (clone MP6-XT22) was from eBioscience. Mouse IgG, Fc and F(ab) were from Jackson Immunoresearch. Wortmannin (W1628) was from Sigma, Chloroquine diphosphate (L10382), Lipofectamine 2000 and Lysotracker Red DND-99 were from Invitrogen. ODN1585, ODN1826, Biotin ODN2216, ODN2216, TLR9 inhibitory ODN (ODN TTAGGG ), ODN TTAGGG control and Imiquimod were from Invivogen.
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